dithiothreitol (Bio-Rad)

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Bio-Rad dithiothreitol
Dithiothreitol, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 978 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reagent+dithiothreitol+dtt/Dithiothreitol+(DTT)+Buffer+Reagent+For+Electrophoresis/bio_rxiv__64898__2026__03__29__715103-163-10-11
Average 96 stars, based on 978 article reviews

dithiothreitol - by Bioz Stars, 2026-09

96/100 stars

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Infection:

Article Title: Characterization of monoclonal antibodies that specifically differentiate field isolates from vaccine strains of classical swine fever virus
Article Snippet: .. Briefly, the supernatant of Sf9 cells infected by recombinant baculovirus for 72 h was collected and treated with SDS loading buffer (Bio-Rad, Shanghai, China) without reducing reagent dithiothreitol (DTT), then loaded in 5%–10% (for E2 protein) or 5%–12% (for E rns protein) SDS-polyacrylamide gel. .. After electrophoresis, the proteins in the gels were transferred onto a nitrocellulose (NC) membrane (Cytiva, Shanghai, China) and subsequently reacted with anti-E2 and E rns mAbs.

Recombinant:

Gel Shift:

Article Title: Structural flexibility of the periplasmic protein, FlgA, regulates flagellar P-ring assembly in Salmonella enterica
Article Snippet: .. Proteins (2 μg) of FlgA and FlgAR113C-S190C for gel-shift assay were prepared in the presence or absence of a reducing reagent dithiothreitol (DTT) and separated in a 4–20% precast polyacrylamide gel (Bio-Rad, Hercules, CA). .. Proteins are visualized with CBB and A DTT(kDa) - -++ FlgA FlgAR113C-S190C B Ab so rb an ce (m AU ) Volume (mL) - DTT C Ab so rb an ce (m AU ) Volume (mL) + DTT 40 30 20 10 0 40 30 20 10 0 7 191513119 17 7 191513119 17 Temperature ( ̊C) Temperature ( ̊C) Cp (k ca l/m ol/ ̊C ) Cp (k ca l/m ol/ ̊C ) D E 20 15 10 5 0 20 15 10 5 0 503020 40 907060 80 FlgA FlgAR113C-S190C 503020 40 907060 80 25 - 20 - 15 - digitized using the ChemiDoc XRS+ system (Bio-Rad, Hercules, CA) (A).

Incubation:

Article Title: The ubiquitin ligase RNF43 downregulation increases membrane expression of frizzled receptor in pancreatic ductal adenocarcinoma.
Article Snippet: RNF43 is a novel tumor suppressor protein and known to be expressed in a multitude of tissue and dysregulated in cancers of these organs including ovarian and colorectal tissues.. RNF43 expression has been shown to be expressed in mutated forms in several pancreatic cell lines.. RNF43, by virtue of being an ubiquitin ligase, has the potential to ubiquitinylate membrane receptors like frizzled that subserves sensing Wnt soluble signals at the cell membrane.

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